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The continued practice of storing tissue and/or cells directly in Liquid Nitrogen LN2 fluid since this widely publicized occurrence in 1996 has slowed. Although this specific type cross contamination has not been reported since 1996, manufacturers of cryogenic storage equipment and components have sought to develop alternatives to direct immersion LN2 liquid nitrogen storage. The objective is for the cryogenic storage system to reliably maintain very constant cryogenic temperatures (Burden et al., 1998) below the glass transition temperature of water [TTGW] at/about -132 degree C. without direct contact with LN2 liquid nitrogen fluid. Also, another important goal is to provide protection of the stored material from any warming sequence which repeatedly goes above the TTGW (Lopaczynski, et al., 2003) caused by working inside the cryogenic freezer chamber, removing selected inventory to reposition, or periodic rise in chamber temperature due to opening a chamber/vessel door or lid.












